TISSUE CULTURE: Methods

Orchid Doctor Propagation

by Robert M. [Bert] Hamilton (Compiler)

Originally published in The Orchid Doctor in 1980 and 1988

Posted by Sys Admin about 8 years ago.

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How to obtain pathogen-free explants; genetic research; hybrids achieved by means of fusion of plant cells; refer to F78-10+; clonal propagation; a manual; refer to OB1-203 to 293,
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Tissue Culture Comments
TISSUE CULTURE: Advantages It has increased the range of choice at the high quality level; but phalaenopsis mericlones take two to three times as long to mature and some never do; paphiopedilum explants produce short-lived callus which seldom yields large batches of normal plantlets. A83-847; for rebuttal, A83-1150 0
TISSUE CULTURE: Everyone Is Doing it in Hawaii Most growers now have their own laboratory; most are no longer using true meristem culture but are doing shoot tip cultures using lateral buds which are easier to excise, they grow faster and proliferate better than true meristerns. F82-61; you still have to make sure you buy the first-rate offspring . RMH 0
TISSUE CULTURE: Mutations They do happen, but usually wi th tissue sub-divisions ; explants from the healthy source plant do not mutate. SA84Jun-68 0

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