Category: Orchid Doctor

FLASKING: Improved Methods of Germination

Author is Robert M. [Bert] Hamilton (Compiler), posted almost 8 years ago Sterile medium is achieved by decontaminating glassware with Physan 20 solution and adding .005% hydrogen peroxide into the medium just before pouring it into flasks; sterilize seed by soaking it 2... Read More

FLASKING: Kitchen Style

Author is Robert M. [Bert] Hamilton (Compiler), posted almost 8 years ago A solution to many problems was found in the use of a clear plastic bag 10 x 12 inches; two holes were cut to fit over the openings of the flasks at the sealed end and the work was done through the... Read More

FLASKING: Methods and Materials

Author is Robert M. [Bert] Hamilton (Compiler), posted almost 8 years ago Apparatus and growth materials required; preparation of seed; aids for simplifying the process; for green capsule and ripe seed; refer to OR81-149; Ca82(6)-4; OBII-243 Read More

FLASKING: Prepared Flasks for Later Use

Author is Robert M. [Bert] Hamilton (Compiler), posted almost 8 years ago Keep them on hand for about one week only; but can be kept for about two months if stoppers are covered with tightly fitting aluminum foil. RMH Read More

FLASKING: Proliferation of Tissue

Author is Robert M. [Bert] Hamilton (Compiler), posted almost 8 years ago Reasons are not clear and no anti-proliferations have been mentioned but certain additives such as banana homogenaate may be useful; for references ref.to A85-1334 Read More

FLASKING: Reflasking

Author is Robert M. [Bert] Hamilton (Compiler), posted almost 8 years ago Airtight capping is not advised as the seedlings can be inhibited by it. A77-314 Read More

FLASKING: Reflasking Alternative

Author is Robert M. [Bert] Hamilton (Compiler), posted almost 8 years ago Use a 2-liter plastic pop bottle; heat the bottom stand in hot water to soften the adhesive and pull it off, cut off the top section of the pop bottle and invert it into the stand portion to form a... Read More

FLASKING: Reflasking with Kappa Carrageenan (?) as a Substitute for Agar

Author is Robert M. [Bert] Hamilton (Compiler), posted almost 8 years ago Extract from red seaweeds is used in the food industry and cosmetics; experiments with it as an orchid medium for flasking culture and tested comparatively with standard agar media. A83-1174 Read More

FLASKING: Replate Medium

Author is Robert M. [Bert] Hamilton (Compiler), posted almost 8 years ago Use the same germinating medium for replating; the Knudson-C formula can be used for both. A70-631 Read More

ACID OR ALKALINE: Changing the Values

Author is Robert M. [Bert] Hamilton (Compiler), posted almost 8 years ago Various means can be used: tufa, a limestone form, can be added to paphiopedilums from limestone cliffs; bark composts acidify with age; peatmoss, pine needles, oak leaves in the mix produce acidit... Read More